СОТРУДНИКИ

Бушуева Ольга Юрьевна - заведующий лабораторией: к.м.н., доцент

Быканова Марина Алексеевна - научный сотрудник, к.м.н.

НАПРАВЛЕНИЯ НАУЧНЫХ ИССЛЕДОВАНИЙ ЛАБОРАТОРИИ

Исследование вариабельности генома человека с использованием молекулярно-генетических методов: выделение ДНК/РНК из образцов крови и тканей; генотипирование ПЦР-ПДРФ, ПЦР-ПДАФ, мультиплексное генотипирование методом MALDI-TOF  масс-пектрометрии, разработка протоколов генотипирования SNP методами ПЦР в режиме «реального времени» с помощью TaqMan-зондов, таргетный анализ генной экспрессии; таргетный анализ метилирования ДНК методом пиросеквенирования.

Молекулярно-генетические исследования мультифакториальных (многофакторных) болезней человека (сердечно-сосудистые, онкологические, неврологические, бронхолегочные): отбор генетических маркеров для генетико-ассоциативных исследований, приоритезация генов, ассоциированных с «предрасположенностью» к болезням; эпигенетические исследования; анализ транскриптома; исследование молекулярных механизмов вовлеченности исследуемых генетических маркеров в развитие заболеваний; изучение механизмов взаимодействия генетических и средовых факторов при мультифакториальных болезнях человека. Биобанкирование образцов биологического материала пациентов с различными мультифакториальными заболеваниями.

ОБЛАСТЬ НАУЧНЫХ ИНТЕРЕСОВ

Генетическая и молекулярная эпидемиология, генетика многофакторных заболеваний, эпигенетика, транскриптомика, молекулярная диагностика.

ИЗБРАННЫЕ ПУБЛИКАЦИИ СОТРУДНИКОВ ЛАБОРАТОРИИ

1. Bushueva OY. Genetic Variants rs1049255 CYBA and rs2333227 MPO are Associated with Susceptibility to Coronary Artery Disease in Russian Residents of Central Russia. Kardiologiia. 2020 Nov 12;60(10):1229. doi: 10.18087/cardio.2020.10.n1229. PMID: 33228505.
2. Bushueva OY, Polonikov AV, Ivanov SV, Ivanov IS, Klesova EY, Tsukanov AV. Variant rs2009262 EFEMP1 Is Associated with the Development of Umbilical Hernia but Not with Postoperative Hernia in Russians. Russian Journal of Genetics. 2020 Jun;56(6):734-8.
3. Korokin M, Gureev V, Gudyrev O, Golubev I, Korokina L, Peresypkina A, Pokrovskaia T, Lazareva G, Soldatov V, Zatolokina M, Pobeda A, Avdeeva E, Beskhmelnitsyna E, Denisyuk T, Avdeeva N, Bushueva O, Pokrovskii M. Erythropoietin Mimetic Peptide (pHBSP) Corrects Endothelial Dysfunction in a Rat Model of Preeclampsia. Int J Mol Sci. 2020 Sep 15;21(18):6759. doi: 10.3390/ijms21186759. PMID: 32942669; PMCID: PMC7554893.
4. Churilin MI, Kononov SI, Luneva YV, Kazanov VA, Azarova IE, Klyosova EY, Bykanova MA, Paschoalini G, Kharchenko AV, Zhabin SN, Bushueva OY. Polymorphisms of intracellular cholesterol transporters genes: relationship to blood lipid levels, carotid intima-media thickness, and the development of coronary heart disease. Russian Journal of Genetics. 2020 Feb;56(2):234-41.
5. Polonikov A, Rymarova L, Klyosova E, Volkova A, Azarova I, Bushueva O, Bykanova M, Bocharova I, Zhabin S, Churnosov M, Laskov V, Solodilova M. Matrix metalloproteinases as target genes for gene regulatory networks driving molecular and cellular pathways related to a multistep pathogenesis of cerebrovascular disease. J Cell Biochem. 2019 Oct;120(10):16467-16482. doi: 10.1002/jcb.28815. Epub 2019 May 5. PMID: 31056794.
6. Polonikov AV, Ponomarenko IV, Bykanova MA, Sirotina SS, Bocharova AV, Vagaytseva KV, Stepanov VA, Azarova IE, Churnosov MI, Solodilova MA. A comprehensive study revealed SNP-SNP interactions and a sex-dependent relationship between polymorphisms of the CYP2J2 gene and hypertension risk. Hypertens Res. 2019 Feb;42(2):257-272. doi: 10.1038/s41440-018-0142-1. Epub 2018 Dec 5. PMID: 30518987.
7. Azarova I, Bushueva O, Konoplya A, Polonikov A. Glutathione S-transferase genes and the risk of type 2 diabetes mellitus: Role of sexual dimorphism, gene-gene and gene-smoking interactions in disease susceptibility. J Diabetes. 2018 May;10(5):398-407. doi: 10.1111/1753-0407.12623. Epub 2018 Jan 2. PMID: 29111615.
8. Sirotina S, Ponomarenko I, Kharchenko A, Bykanova M, Bocharova A, Vagaytseva K, Stepanov V, Churnosov M, Solodilova M, Polonikov A. A Novel Polymorphism in the Promoter of the CYP4A11 Gene Is Associated with Susceptibility to Coronary Artery Disease. Dis Markers. 2018 Feb 1;2018:5812802. doi: 10.1155/2018/5812802. PMID: 29484037; PMCID: PMC5816861.
9. Tikunova E, Ovtcharova V, Reshetnikov E, Dvornyk V, Polonikov A, Bushueva O, Churnosov M. Genes of tumor necrosis factors and their receptors and the primary open angle glaucoma in the population of Central Russia. Int J Ophthalmol. 2017 Oct 18;10(10):1490-1494. doi: 10.18240/ijo.2017.10.02. PMID: 29062765; PMCID: PMC5638967.
10. Polonikov A, Kharchenko A, Bykanova M, Sirotina S, Ponomarenko I, Bocharova A, Vagaytseva K, Stepanov V, Bushueva O, Churnosov M, Solodilova M. Polymorphisms of CYP2C8, CYP2C9 and CYP2C19 and risk of coronary heart disease in Russian population. Gene. 2017 Sep 5;627:451-459. doi: 10.1016/j.gene.2017.07.004. Epub 2017 Jul 4. PMID: 28687336.
11. Reshetnikov E, Zarudskaya O, Polonikov A, Bushueva O, Orlova V, Krikun E, Dvornyk V, Churnosov M. Genetic markers for inherited thrombophilia are associated with fetal growth retardation in the population of Central Russia. J Obstet Gynaecol Res. 2017 Jul;43(7):1139-1144. doi: 10.1111/jog.13329. Epub 2017 May 19. PMID: 28544373.
12. Polonikov AV, Bushueva OY, Bulgakova IV, Freidin MB, Churnosov MI, Solodilova MA, Shvetsov YD, Ivanov VP. A comprehensive contribution of genes for aryl hydrocarbon receptor signaling pathway to hypertension susceptibility. Pharmacogenet Genomics. 2017 Feb;27(2):57-69. doi: 10.1097/FPC.0000000000000261. PMID: 27977510.
13. Polonikov AV, Samgina TA, Nazarenko PM, Bushueva OY, Ivanov VP. Alcohol Consumption and Cigarette Smoking are Important Modifiers of the Association Between Acute Pancreatitis and the PRSS1-PRSS2 Locus in Men. Pancreas. 2017 Feb;46(2):230-236. doi: 10.1097/MPA.0000000000000729. PMID: 27846138.
14. Kirilenko MY, Tikunova EV, Sirotina SS, Polonikov AV, Bushueva OY, Churnosov MI. Izuchenie assotsiatsiĭ geneticheskikh polimorfizmov faktorov rosta s razvitiem pervichnoĭ otkrytougol'noĭ glaukomy [Studying the association between genetic polymorphism of growth factors and the development of primary open-angle glaucoma]. Vestn Oftalmol. 2017;133(3):9-15. Russian. doi: 10.17116/oftalma201713339-15. PMID: 28745651.
15. Polonikov A, Bykanova M, Ponomarenko I, Sirotina S, Bocharova A, Vagaytseva K, Stepanov V, Churnosov M, Bushueva O, Solodilova M, Shvetsov Y, Ivanov V. The contribution of CYP2C gene subfamily involved in epoxygenase pathway of arachidonic acids metabolism to hypertension susceptibility in Russian population. Clin Exp Hypertens. 2017;39(4):306-311. doi: 10.1080/10641963.2016.1246562. Epub 2017 May 17. PMID: 28513222.
16. Sorokin AV, Kotani K, Bushueva OY. Association of matrix metalloproteinase 3 and γ-glutamyltransferase 1 gene polymorphisms with the cardio-ankle vascular index in young Russians. Cardiol Young. 2016 Aug;26(6):1238-40. doi: 10.1017/S104795111600069X. Epub 2016 May 10. PMID: 27161754.
17. Samgina TA, Bushueva OY, Nazarenko PM, Polonikov AV. Association of the HindIII Lipoprotein Lipase Gene Polymorphism with the Development of the Non-Biliary Acute Pancreatitis: a Pilot Study. Bull Exp Biol Med. 2016 May;161(1):79-82. doi: 10.1007/s10517-016-3350-1. Epub 2016 Jun 6. PMID: 27270932.
18. Bushueva OY, Ivanov VP, Ryzhaeva VN, Ponomarenko IV, Churnosov MI, Polonikov AV. Assotsiatsiya polimorfizma -844G>A gena katalazy s povyshennym riskom razvitiya arterial'noi gipertonii u kuril'shchikov [Association of the -844G>A polymorphism in the catalase gene with the increased risk of essential hypertension in smokers]. Ter Arkh. 2016;88(9):50-54. Russian. doi: 10.17116/terarkh201688950-54. PMID: 27735913.
19. Zvyagina MV, Mal GS, Bushueva OY, Alymenko MA, Bykanova MA, Letova IM, Gribovskaya IA, Churnosov MI, Solodilova MA, Polonikov AV. [ESTIMATING THE EFFECTIVENESS OF HYPOLIPIDEMIC THERAPY WITH ROSUVASTATIN IN PATIENTS WITH CORONARY HEART DISEASE DEPENDING ON THE GENOTYPE OF LIPOPROTEIN LIPASE]. Eksp Klin Farmakol. 2016;79(1):15-9. Russian. PMID: 27159952.
20. Polonikov AV, Ushachev DV, Ivanov VP, Churnosov MI, Freidin MB, Ataman AV, Harbuzova VY, Bykanova MA, Bushueva OY, Solodilova MA. Altered erythrocyte membrane protein composition mirrors pleiotropic effects of hypertension susceptibility genes and disease pathogenesis. J Hypertens. 2015 Nov;33(11):2265-77. doi: 10.1097/HJH.0000000000000699. PMID: 26335431.
21. Bushueva OY, Bulgakova IV, Ivanov VP, Polonikov AV. Association of Flavin Monooxygenase Gene E158K Polymorphism with Chronic Heart Disease Risk. Bull Exp Biol Med. 2015 Oct;159(6):776-8. doi: 10.1007/s10517-015-3073-8. Epub 2015 Oct 31. PMID: 26519273.
22. Bushueva O, Solodilova M, Ivanov V, Polonikov A. Gender-specific protective effect of the -463G>A polymorphism of myeloperoxidase gene against the risk of essential hypertension in Russians. J Am Soc Hypertens. 2015 Nov;9(11):902-6. doi: 10.1016/j.jash.2015.08.006. Epub 2015 Aug 21. PMID: 26431910.
23. Sorokin AV, Kotani K, Bushueva OY, Polonikov AV. Antioxidant-related gene polymorphisms associated with the cardio-ankle vascular index in young Russians. Cardiol Young. 2016 Apr;26(4):677-82. doi: 10.1017/S104795111500102X. Epub 2015 Jun 17. PMID: 26081749.
24. Bushueva OY, Stetskaya TA, Korogodina TV, Ivanov VP, Polonikov AV. [The combined effect of E298D polymorphism of the endothelial nitric oxide synthase gene and smoking on the risk of cerebral stroke]. Genetika. 2015 Feb;51(2):256-62. Russian. PMID: 25966592.
25. Bushueva OY, Stetskaya TA, Korogodina TV, Ivanov VP, Polonikov AV. Issledovanie vzaimosvyazi polimorfizmov HindIII gena LPL i Taq1b gena CETP s riskom razvitiya aterotromboticheskogo insul'ta u zhitelei Tsentral'noi Rossii [Investigation of the association between the HindIII polymorphism of the LPL gene and the Taq1b polymorphism of the CETP gene with the risk of atherothrombotic stroke in the dwellers of Central Russia]. Ter Arkh. 2015;87(8):86-91. Russian. doi: 10.17116/terarkh201587886-91. PMID: 26824821.
26. Vasil'eva Yu I, Bushueva OY, Zhabin SN, Ivanov SV, Polonikov AV. [SMOKING AS A TRIGGER FACTOR IN THE DEVELOPMENT OF DIABETIC ANGIOPATHY OF LOWER EXTREMITIES IN MEN WITH METHYLENETETRAHYDROFOLATEREDUCTASE 677TT GENOTYPE]. Klin Med (Mosk). 2015;93(7):45-9. Russian. PMID: 26596059.
27. Bushueva OY, Stetskaya TA, Polonikov AV, Ivanov VP. Sviaz' polimorfizma 640a>g gena cyba s riskom razvitiia ishemicheskogo insul'ta u russkikh v Tsentral'noĭ Rossii [The relationship between polymorphism 640A>G of the CYBA gene with the risk of ischemic stroke in the population of the Central Russia]. Zh Nevrol Psikhiatr Im S S Korsakova. 2015;115(9 Pt 2):38-41. Russian. doi: 10.17116/jnevro20151159238-41. PMID: 26525933.
28. Bushueva OY, Stetskaya TA, Korogodina GV, Ivanov VP, Polonikov AV. [GENDER-SPECIFIC DIFFERENCES OF ENDOTHELIAL NITRIC OXIDE SYNTHASE E298D POLYMORPHISM AND THE RISK OF STROKE]. Klin Med (Mosk). 2015;93(2):34-40. Russian. PMID: 26117916.
29. Sorokin A, Kotani K, Bushueva O, Taniguchi N, Lazarenko V. The cardio-ankle vascular index and ankle-brachial index in young russians. J Atheroscler Thromb. 2015;22(2):211-8. doi: 10.5551/jat.26104. Epub 2014 Sep 17. PMID: 25230696.
30. Stetskaia TA, Bushueva OIu, Bulgakova IV, Vialykh EK, Shuteeva TV, Biriukov AE, Ivanov VP, Polonikov AV. [Association of T174M polymorphism of the angiotensinogen gene with the higher risk of cerebral stroke in women]. Ter Arkh. 2014;86(12):66-71. Russian. doi: 10.17116/terarkh2014861266-71. PMID: 25804043.
31. Bushueva O, Solodilova M, Churnosov M, Ivanov V, Polonikov A. The Flavin-Containing Monooxygenase 3 Gene and Essential Hypertension: The Joint Effect of Polymorphism E158K and Cigarette Smoking on Disease Susceptibility. Int J Hypertens. 2014;2014:712169. doi: 10.1155/2014/712169. Epub 2014 Aug 27. PMID: 25243081; PMCID: PMC4163302.
32. Polonikov A, Vialykh E, Vasil'eva O, Bulgakova I, Bushueva O, Illig T, Solodilova M. Genetic variation in glutathione S-transferase genes and risk of nonfatal cerebral stroke in patients suffering from essential hypertension. J Mol Neurosci. 2012 Jul;47(3):511-3. doi: 10.1007/s12031-012-9764-y. Epub 2012 Apr 15. PMID: 22528457.